Journal: Journal of Biological Chemistry
Article Title: Plasminogen Activator Inhibitor-1 Regulates Tumor Growth and Angiogenesis
doi: 10.1074/jbc.m105980200
Figure Lengend Snippet: FIG. 3. Immuno-analysis of vessel architecture in M21 tumors. Tumor sections treated with PBS (A, D, G), partially inactivated PAI-1 (B, E, H), or fully active PAI-1 (C, F, I) were stained for the following: immunohistochemical staining for collagen IV (A–C), immunohisto- chemical staining for smooth muscle cell actin (D–F), immunofluores- cence analysis of vitronectin (green) (G–I), endothelial cells (red), and tumor cells (blue). The original magnification for all panels is 400.
Article Snippet: For immunofluorescence staining of tumor sections, slides were treated as above and then double stained with rabbit anti-murine vitronectin (Molecular Innovations) and rat anti-murine PECAM-1 (PharMingen) and detected with Flour 488 goat anti-rabbit IgG (Molecular Probes) and Texas Red rat anti-mouse IgG (Molecular Probes).
Techniques: Staining, Immunohistochemical staining